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2.6 Pulldown assays Pulldown assays were performed as previously described ( 2 , 1% TritonX100 (cat #T8787, Sigma Aldrich Chemicals Co), 1 mM Phenylmethanesulfonyl fluoride [PMSF] (cat #P7626, Sigma Aldrich Chemicals Co), cOmplete EDTA-free protease inhibitor cocktail (cat # 11873580001, Roche, Basel, Switzerland), 2 mM Sodium orthovanadate [Na 3 VO 4 ] (cat #S6508, Sigma Aldrich Chemicals Co), 10 mM Sodium pyrophosphate tetrabasic decahydrate [NaPPi] (cat #S6422, Sigma Aldrich Chemicals Co) and 80 mM -glycerophosphate (cat #14405, Cayman Chemical Company