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As ROS/NO scavengers alleviated both the formation of SI-induced actin punctate foci and also activation of a DEVDase/caspase-3-like activity (Wilkins et al., 2011)

The difference is purely in how many total doses you extract before needing a new vial

This is due in part to the use of fluorescent resonance energy transfer (FRET), fluorophores were carefully selected such that, when the fluorophores are paired, activation of the donor fluorophore causes a highly efficient energy transfer to the acceptor fluorophore, producing a large emission from the acceptor fluorophore, this effect diminishes exponentially as the distance between the fluorophores increases