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At least ten separate images per treatment were counted, measured in diameter, and scored as dispersed or not dispersed based on central hollowing of microcolony architecture

A framework for understanding gene expression plasticity and its influence on stress tolerance

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The degree of synovial fibrosis was evaluated by calculating the positive area of collagen fibers and the percentage of collagen I positive areas with ImageJ (National Institutes of Health, USA, available at TUNEL staining The degree of apoptosis was detected by TUNEL staining in synovial tissues