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The problem: glutathione levels decline with age, stress, poor diet, and environmental toxins

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Western blot analysis and antibodies Total protein of lung tissue samples was extracted by using T-PER tissue protein extraction reagent (Thermo Scientific), while total cellular proteins were extracted using Radioimmunoprecipitation Assay (RIPA) lysis buffer (Beyotime, China) and phenylmethylsulfonyl fluoride (Sigma-Aldrich)