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Functionally, glutathione participates in a wide range of biochemical processes, including: Regulation of reactive oxygen species (ROS) Support of cellular detoxification mechanisms via conjugation pathways Maintenance of protein thiol status and intracellular redox signaling Involvement in mitochondrial function and metabolic regulation Due to its broad biological relevance, glutathione is extensively utilized in in vitro studies, oxidative stress models, and biochemical pathway research , particularly in fields such as molecular biology, toxicology, and cellular physiology