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As a signaling intermediate, elevated ROS levels can affect growth factor bioavailability by preventing extracellular matrix (ECM) synthesis, affecting bioavailability, participating in degradation of ECM components, promoting MMP production, and inducing chondrocyte death (40)

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Moreover, mTOR kinase activity may be suppressed by the activation of AMPK itself 11

These results are consistent with the reported role of hinge-region serine phosphorylation in the cytoplasmic retention of nuclear hormone receptors 67