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R.et al (2006)

3T3-L1 cells were cultured in 60 mm diameter dishes (8.4104 cells/dish) and treated with NNMT inhibitor dissolved in culture media with/without adipogenic factors (1 mM IBMX, 1 M dexamethasone, 10 g/ml of insulin) during each of the scheduled media changes during the differentiation process (described above)