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For Western blot analysis, 30 g protein per sample were mixed with loading buffer, denatured at 95 C for 5 min (PDHA1 and pPDHA1) or at 37 C for 20 min (OXPHOS complexes) and separated on a TGX Stain-Free Precast gradient gel (Bio-Rad Laboratories GmbH, Germany)

[DOI] [PubMed] [Google Scholar] 142.Bort A., Alvarado-Vazquez P.A., Moracho-Vilrriales C., Virga K.G., Gumina G., Romero-Sandoval A., Asbill S

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Similarly, Nivolumab restores T cell functions by blocking the PD-1 receptor