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Funding This work is supported by the Natural Science Project of Sichuan Provincial Science and Technology Department (24NSFSC8042)

For the RBC separation, the blood buffy coat was washed three times with PBS (10 mM phosphate- buffered saline, pH 7.4)

For acute injuries with significant pain and swelling, some practitioners and experienced users suggest a higher loading approach during the first 3-5 days: 0.5 mg twice daily (1 mg total) before reducing to maintenance dosing