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The reasons behind this are incredibly complex, but one of the most widely accepted explanations is called the free radical theory of aging [1]

Detailed assessment of cytoplasmic glutamate concentrations that are needed to maintain mEPSC amplitudes in an invasive situation (without presynaptic whole-cell recordings) revealed that the presynaptic glutamate concentration at the calyx of Held is ~1 mM (Ishikawa et al., in vitro (Naito and Ueda, 1996

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