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Key Points The sculpting of neuronal connections during brain development depends on a precise balance between inhibition and excitation an excess of GABA (-aminobutyric acid)-mediated inhibition might prevent growth or synapse formation, whereas an excess of glutamatergic excitation could lead to toxicity

2 cells are mouse-derived multipotent neural stem cells isolated from cerebellum, which were immortalized by avian myelocytomatosis viral-related oncogene (v-myc) transfection (Snyder et al., 1992)