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It was not until a genetically engineered strain of Pseudomonas denitrificans was used, in which eight of the genes involved in the biosynthesis of the vitamin had been overexpressed, that the complete sequence of methylation and other steps could be determined, thus fully establishing all the intermediates in the pathway

These may last forever and include nerve damage, thinking problems, and anaemia

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The term non-hydrogen substituent refers to a substituent that is not made up solely of hydrogen