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Had the second 3 weeks later

Quantification of NAD/NADH and NADP+/NADPH ratios Cells from overnight cultures of H99 were washed twice with PBS prior to quantifying concentration on a Countess II FL automated cell counter

This chemical modification, combined with optimized liquid chromatography conditions, allows for the complete resolution of isobaric and isomeric compounds, such as leucine and isoleucine, enabling their confident quantification even in a Selected Ion Recording (SIR) mode using a single quadrupole MS system (Supplementary Fig

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