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AS3MT automethylation requires four cysteine residues: C33, C62, C157, and C207 To identify the automethylated residues of mouse AS3MT in vivo, Flag-epitope tagged AS3MT was immunoprecipitated from stably transfected AML12 hepatocyte cells and the precipitates were digested with trypsin and subjected to mass spectrometry (MS/MS) for peptide detection (Fig

2 Materials and methods We conducted an extensive systematic literature search to better understand the methods and protocols employed in preclinical studies for TBI treatment

doi: 10.1016/j.molcel.2020.07.026 69 DuSSChenGWYangPChenYXHuYZhaoQQet al

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