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Distinction from other forms of cell death: Disulfide death is independent of apoptosis, ferroptosis, necroptosis, and cuproptosis, with its specificity relying on disulfide bond cross-linking, differentiating it from lipid peroxidation in ferroptosis or copper ion toxicity in cuproptosis

Subsequently, cAMP response element binding protein (CREB) and Nuclear factor kappa-B (NF-B) are activated by CaMKII and PKC, and the Nuclear factor of activated T cells (NFAT) in the cytoplasm is activated and dephosphorylated by CalN

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In the second step, the complexes of enzyme fragments and substrate were screened to find the enzyme inhibitor