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The critical design choice was the C-terminal PGP extension: Pro-Gly-Pro provides primary resistance to proteolytic degradation, extending the compound's half-life from the minutes range of native ACTH(4-7) to approximately several hours, enabling meaningful and sustained CNS activity following administration

Following a 15-min incubation period, living cells exhibited the ability to absorb and bind the neutral red dye within their lysosomes

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