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Liver disease, as vitamin B12 metabolism occurs in the liver and may be affected 6

The plates were incubated for 2 h at 37C and the absorbance at 450 nm wavelength, which was obtained as the optical density (OD) value, was measured with a Multiskan SkyHigh automatic microplate reader (Thermo Fisher Scientific, Inc.)

The ALSSQOL is validated in patients with ALS and correlates with ALSFRS-R scores, as well as other measures of quality of life [58, 59]

Since oxidative neuronal death is one of the major neurodegenerative mechanisms in PD, the antioxidant capacity of ibuprofen along with its antidepressant effects have also been discussed