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Optimization strategies for combination therapies The optimization of combination therapies should be guided by three synergistic principles: complementary metabolic pathway targeting, spatiotemporal coordination, and metabolic-immune crosstalk regulation

After solubilization of neutral-red stain with 0.1 mL of acidified alcohol (10% acetic acid, 40% ethanol in distilled water) the absorbance was measured in a microplate reader at 550 nm

Given the role of NAD + in modulating the processes described above, NAD + -boosting interventions are being investigated for supporting mitochondrial homeostasis