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studies in mice have shown that long-term oral antifungal medications can exacerbate colitis and worsen allergic airway disease [151]

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Additionally, Dolly the sheep was the first animal to ever be successfully cloned from cultured somatic cells 27 , making it possible to use the somatic cell nuclear transfer (SCNT) technique for both biomedical and agricultural applications of sheep 28

Cell viability was measured by assaying mitochondrial activity using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) transformation, as previously described ( Measurement of Intracellular Ca 2+ Concentration Intracellular calcium concentration ([Ca 2+ ] i ) was measured using the fluorescent indicator fura-2