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Table II [i] MASLD metabolic dysfunction-associated steatotic liver disease, ROS reactive oxygen species, MDA malondialdehyde, 4-HNE 4-hydroxynonenal, 8-OHdG 8-hydroxy-2-deoxyguanosine, ox-LDL oxidized low-density lipoprotein, NOX NADPH oxidase, CYP2E1 cytochrome P450 2E1, TGF- transforming growth factor , UCP2 uncoupling protein 2

Phase I detoxification uses cytochrome P450 enzymes to break down toxins, but this process often creates highly reactive, intermediate metabolites that are actually more toxic than the original substance

RNA quality and integrity were measured with the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer, according to manufacturers instructions

Plasma levels of ferroptosis and OS markers, including iron, ferritin (FE), transferrin (TF), glutathione peroxidase 4 (GPX4), long-chain acyl-CoA synthetase 4 (ACSL4), glutathione (GSH), sirtuin 1 (SIRT1), nuclear factor erythroid 2-related factor 2 (Nrf2), malondialdehyde (MDA), and superoxide dismutase (SOD) were measured