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Construction of inserts for ligation was done using the melt and reanneal method as per ref

The fractions containing reduced glutathione are concentrated by nanofiltration and by distillation under vacuum, after which the product is isolated by spray-drying as described in example 1, with input air at about l45C and output temperature of about 98C

Parkin deficiency compromises mitochondrial function in Drosophila [39], mice [40], and iPSC-derived neurons from individuals with Parkin PD [41]