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HUVECs and NIH 3T3 cells in Roswell Park Memorial Institute (RPMI) 1640 and HaCaT in Dulbeccos Minimum Essential Medium (DMEM)/F-12 medium were cultured in the indicated media supplemented with 10% fetal bovine serum (FBS) and maintained at 37C in a humidified environment with 5% CO 2

Serum aspartate aminotransferase and alanine transaminase levels were measured at 10 h post-APAP administration

Initial signs of response typically appear by week 3-4, with meaningful results developing through weeks 5-8

The identification of nongenetic factors underlying regionspecific SOD1 misfolding and deposition in SOD1 linked familial ALS, which may also contribute to wildtype SOD1 misfolding in sporadic ALS, idiopathic Parkinson's disease and potentially Alzheimer's disease will significantly improve our understanding of the biology of this protein