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Lysates of Raw264.7 cells were prepared with lysis buffer containing 50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 10% glycerol, 150 mM NaCl, 1% NP-40, and protease/phosphatase inhibitors (Thermo Fisher Scientific) and incubated with the peptide-conjugate resins for 1 h at 4C

10.1016/j.nxnano.2024.100129 130 KusiJ.ScheuermanP

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This shuts down melatonin production for the day, increases melatonin production for the next night, and improves sleep quality.[ref] Brighter lights during the day when working inside can increase melatonin at night.[ref] Eat on a regular schedule