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RNA samples were treated with the TURBO DNA-free kit (Applied Biosystems, Foster City, CA), further purified through an RNease kit (Qiagen) and quantified with a NanoDrop-1000 (NanoDrop Technologies Inc., Wilmington, DE)

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Cytokine-induced activation of nuclear factor-kappa B is inhibited by hydrogen peroxide through oxidative inactivation of IkappaB kinase

The connection between glutamine metabolism and mTORC1 present additional connection branches, as glutamine also plays a role in autophagy-induced mTORC1 restoration during amino acid starvation [91]