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While peroxidases were known at the time, and their possible involvement in ascorbate oxidation previously noted (Mapson and Goddard 1951), the physiological functions of such enzymes were considered to be as much in H 2 O 2 -dependent oxidations as reductant-dependent H 2 O 2 removal

Determination of Free and Bound SA The third true leaves (counted from below) of 6070 days-old tobacco plants were analyzed to determine free and bound forms of SA essentially as described by Analyses of TMV Accumulation and Tobacco Defense Gene Expression To analyze TMV accumulation and tobacco defense gene expression 200 mg fresh plant leaf tissue per sample was collected and grounded in liquid nitrogen

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