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The extensor digitorum longus muscles were carefully dissected from WT and VECAD IGF- 1R KD mice while continuously superfused with oxygenated KrebsHenseleit buffer (95% O, 5% CO, pH 7.4, 37 C) to maintain physiological conditions

[DOI] [PMC free article] [PubMed] [Google Scholar] 21.Singh R.K., Chang H.W., Yan D., Lee K.M., Ucmak D., Wong K., Abrouk M., Farahnik B., Nakamura M., Zhu T.H., et al

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(2021a) used a cast net with a mesh size of 1015 mm to collect macroinvertebrates and large and small fin fishes, which were preserved in a sealed container with ice for later analysis