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Yoshida M, Ishigaki K, Nagai T, Chikyu M, Pursel VG: Glutathione concentration during maturation and after fertilization in pig oocytes: relevance to the ability of oocytes to form male pronucleus

The treatments and their mechanisms of action have been mentioned in Table 2

To verify the absence of bacteria, flies from two different germ-free vials were crushed in ~ 200uL of sterile dH2O in an aseptic environment, then plated on blood agar