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NAC use is based on plotting APAP levels, on the Rumack-Matthew nomogram: Image from Wikipedia [Link is HERE] Intravenous NAC therapy includes: An initial dose of 150 mg/kg over 1 hour, A second dose of 50 mg/kg over 4 hours, And a final infusion of 100 mg/kg over 16 hours

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Peak fractions with RNH1(D210N)-GFP-Tat11-His6, determined by SDS-PAGE, were pooled and diluted with buffer B [50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 10% (v/v) glycerol, and 1 mM dithiothreitol (DTT)] to a final concentration of NaCl of 150 mM