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Assembly of the three PCR products two from both flanking regions of the target gene, one from the antibiotic resistance gene was accomplished using overlap extension PCR with primers hybridizing at both extreme ends, and the resulting DNA fragment was purified by agarose gel electrophoresis

RA was diagnosed based on the American College of Rheumatology (ACR) guidelines and the severity of rheumatoid arthritis (RA) was assessed according to the Disease Activity Score 28 (DAS 28)

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This test has recently been described in detail and applied to patients suffering from either long COVID or ME/CFS (306)