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Sensorgram data were processed and analyzed using the Biacore T200 Evaluation Software (version 3.2.1) with a 1:1 Langmuir binding model to calculate apparent binding affinity (KD) values

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These negative effects of GPx1 overexpression, however, are associated with prolonged upregulation of GPx1 or its activity, as found in GPx1 transgenic models, or in models with sustained activation of Nrf2 to foster increased production and recycling of GSH

2.2 Methionine and mTOR pathway The mTOR serves as a central molecular hub that positively governs anabolic processes while simultaneously exerting negative regulation on certain catabolic pathways (Xie and Proud, 2013)