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Description

sgRNA complexes were synthesized by mixing equal volumes of crRNA (2 l, 160 M in nuclease-free water) and tracrRNA (2 l, 160 M) and incubated at 37 C for 30 min before Cas9 (2.7 l, 10 g l 1

Galloway, S

Symptoms of mercury poisoning include a deteriorated nervous system, impaired motor system, impaired hearing, speech, tremors, headaches, and other symptoms