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These subtypes differ not only in their genetic profiles but also in their clinical outcomes, with copy-number high tumors often harboring P53 mutations or the presence of frequent FBXW7 and PPP2R1A mutations and increased levels of cell cycle dysregulation (due to alterations in CCNE 1, PIK3CA, MYC, and CDKN2A) showing the worst prognosis 4

Finally, after nuclear staining with DAPI for 5 min, the NPCs were washed three times with PBS and imaged with a fluorescence microscope

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Du H, Yan SS