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Total RNA was immediately extracted from 1 ml of pelleted cells with 1 ml of TRIzol reagent and purified using the spin columns as described above

[344] also reported that the use of GA altered the diversity of the gut microbiota and activated the bile acid metabolic pathway, being this fact associated with enhanced ILC3 cells in mesenteric lymph nodes and lamina propria accompanied by reductions in TNF-, IFN-, IL-6, IL-17A, and IL-23, and elevations in IL-10, TGF- and IL-22

The cholesterol diet consisted of the same chow supplemented with 0.5% Cholesterol (Sigma Company, 95% of purity), dissolved in 5% of corn oil containing 15% of saturated fatty acids and 85 % of unsaturated fatty acids approximately, (Mazeite, Refinadora de Maz Venezolana C.A., Aragua, Venezuela)