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Animals were crashed by homogenization (1:10, w:v in 50 mM potassium phosphate buffer, pH 7.0, 0.5 mM EDTA, 1 mM PMSF) and centrifuged (8,000 g, 15 min, 4 C)

Some of the labeling scenarios required to solve the HIV Nef structure are presented in Table 1 of Grzesiek et al

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