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VD was associated with a significant reduction in TGF-1 expression, as well as the activation of SMAD-2 and SMAD-3

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Future research should focus on clinical trials to establish efficacy, improve bioavailability, and create standardized formulations of OSCs for cardiovascular disease prevention and management

The Bradford assay was used to determine protein concentration of cell extracts, from there samples with the same amount of protein were separated using SDS-PAGE technology and transferred onto a PVDF membrane