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ASC oligomerization For ASC oligomer cross-linking, cells were lysed in buffer (0.5% Triton 100, 20 mM HEPES-KOH, pH 7.5, 150 mM KCl, and complete protease and phosphatase inhibitor cocktail) on ice by syringing 10 times through a G26 needle

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It is required for the biosynthesis of proteins, co-enzymes, prosthetic groups, vitamins, amino acids like Cys and Met, GSH, and secondary metabolites such as GSL and sulfoflavonoids