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To determine the accurate concentrations of [ 15 N 13 ]-CNCbl used for each extraction, a calibrator solution comprising unlabeled CNCbl (50-100 ng/mL) and labeled [ 15 N 13 ]-CNCbl (50-100 ng/mL) was routinely prepared and measured by LC-MS/MS

(B) Retinal fundus photograph of a normal retina where the nerve fiber layer appears as an even, white area composed of fibers sweeping towards the optic nerve head

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