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Experiments were conducted using DIO male C57BL/6J mice (Janvier Labs) kept on a HFHS diet (58 kcal% fat, D12331i, Research Diets) from 8 weeks of age

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A total of 235 unique proteins were identified across the three groups and quantified using DIA-NN, focusing on prototypic peptides uniquely mapped to individual proteins (Supplementary Table 1)

All authors contributed to the interpretation of data, and revised the manuscript