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The insoluble portion was subsequently removed by centrifugation and the supernatant was incubated with Ni-NTA resin (GE Healthcare) for 2 h batch binding at 4 C

Moreover, the R17 Cagri- E14 Cagri ionic lock was present in the bypass conformation but absent in the CT-like conformation in both CagriBB-bound structures, consistent with a higher energy requirement to form the CT-like conformation (Supplementary Fig

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