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Crit Rev Clin Lab Sci (2008) 45:123

Aim To determine oxidative/antioxidant status in plasma of patients with different stages of colorectal carcinoma using malondialdehyde concentration, superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activities and distribution of superoxide dismutase isoforms

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This helps to distract the brain from the immediate injection pain, flattens the area so it doesnt leave the Botox as a small lump, and helps to slightly spread the area of coverage for the Botox to work