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The pathophysiology of AD is complex, combining genetic predisposition and impaired epidermal barrier function with an unbalanced inflammatory response to environmental factors (1, 18)

Moreover, simplified experimental paradigms often fail to capture the complex, multifactorial nature of human neurodegeneration, including chronic inflammation, metabolic dysfunction, and cumulative injury [569571]

Filtration through a size exclusion column to remove the excess of reactive keto-enal before the addition of cysteine-containing molecules to the functionalised AON proved to be sufficient, yielding the desired conjugate