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GLUTag cells, originally derived by single-cell cloning of a proglucagon-SV40 large T antigeninduced tumor, were routinely maintained in high-glucose (25 mmol/L) DMEM (Gibco) supplemented with 10% FBS (44)

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SODs, as enzymes characterized by very fast second-order constants, facilitate the encounter of enzymes with substrates during enzyme-catalyzed disproportionation reactions by the presence of a strong attraction electric field generated by the charge on the surface of the enzyme or by reducing the dimensionality of the search process (electrostatic promotes diffusion) [10]