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Cells were maintained in Dulbeccos Modified Eagle Medium (EMEM) supplemented with 10% (v/v) fetal bovine serum (FBS) and antibiotics mixture (penicillin, streptomycin, amphotericin B) in standard culture conditions (5% CO 2 , 37 C, 95% humidity)

V dnen spolenosti jsme ve velk me vystaveni psoben volnch radikl (oxidanmu stresu) v dsledku stresu, uvn lk, alkoholu a drog, psoben kodlivin a zpracovanch potravin

The membranes were washed three times for 5 min with PBST and visualized by scanning at 700 nm and 800 nm with Odyssey XF (LI-COR) imaging system

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