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MCF10A cells were obtained from American Type Culture Collection (Manassas, VA, United States), and maintained in DMEM/F12 (Nakarai Tesque Inc., Kyoto, Japan) supplemented with 20 ng/mL of epidermal growth factor (EGF) (Peptide Institute Inc., Osaka, Japan), 100 ng/mL of cholera toxin (Sigma, St

Tamoxifen was prepared at 20mg/mL in sterile corn oil (Sigma C8267) and shaken at 32C until in solution

This process promotes ubiquitination and degradation of Toll-like receptor 4 (TLR4), thereby inhibiting the NF-B signaling pathway and reducing IL-1 expression in M1 macrophages, ultimately alleviating lung inflammatory responses (90)