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2.6 Cerebral infarct volume At 72 h following reperfusion, the brains were frozen at 20C for 20 min and then sliced into six sections along the coronal plane, immersed in 2% (v/v) TTC (batch 23206277, Sigma, St Louis, MO, United States), stained at 37C without light for 15 min to visualize the infarct volume, and fixed in 4% paraformaldehyde solution (batch 22182401, Biosharp, Anhui, China)

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Comparative profiling of skeletal muscle models reveals heterogeneity of transcriptome and metabolism