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Viral vectors AAV.PHP.eB vectors were prepared at PackGene Biotechnology (Guangzhou, China) respectively with the transgene cassettes: CMV-driven mCherry (titer: 1 10 13 GC/mL), Gpx1- MYC (1 10 13 GC/mL) , Gpx4- HA (1.28 10 13 GC/mL), and HC-promoter ( Rbm24 -Eh3 enhancer following with Hsp68 mini-promoter sequences)-driven Cre (1 10 13 GC/mL)

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Studies have confirmed (121, 122) that p53 can induce ferroptosis not only by inhibiting SLC7A11 activity but also by increasing CDKN1A expression or decreasing DPP4 activity, suggesting the dual regulatory role of p53 in the regulation of ferroptosis