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ROS detection may occur at the apoplasticplasma membrane interface such as through NADPH oxidase (RBOH) activation which subsequently triggers intracellular signaling cascades that alter gene expression

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In order to utilize pLEXSY_I-blecherry3 as a plasmid backbone for Modular Cloning, any pre-existing BsaI recognition sites had to be removed from the plasmid to avoid fragmentation of the backbone during cloning