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Concurrently, LPS significantly elevated TNF- and IL-6 levels in BALF, which were markedly decreased in mice pretreated with 10 g/g GHK-Cu

Following incubation for 48 or 72 h, cells were washed with ice-cold ammonium acetate (150 mM, pH 7.3)

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Oxidative Stress Is Not the Primary Mediator of Disease Pathology While tissue injury often increases ROS production and contributes to further damage, the primary pathological driver may not be oxidative stress, as it occurs in many cases secondary to other initiating factors (Figure 7)