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GGT1 mRNA (a) (n = 2 technical replicates representative of one experiment) and GGT activity (b) (n = 8 technical replicates from 3 independent experiments) in control (208 M cystine) medium were determined

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Standard assays of GST activity The activities of the GST stocks were assayed at 30 C with the standard substrates, 1 mM CDNB (stock solution in ethanol) and 1 mM GSH in 0.1 M sodium phosphate buffer pH 6.5, containing 1 mM EDTA, and the formation of the product S-2,4-dinitrophenyl-glutathione (DNP-SG) was followed spectrophotometrically at 340 nm 23