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Among different cell types, red blood cells (RBCs) have a strong dependence on G6PDH due to their lack of mitochondria, making G6PDH an essential NADPH source for preventing RBCs against oxidative damage caused by reactive oxygen species (ROS) (Prankerd, 1961

For fluorescent labeling, we suggest negatively quantifying the amount of ligand bound by measuring ligand remaining in the coupling supernatant (compared to the original sample), rather than directly measuring the ligands on the beads